Salmonella PCR Igikoresho cyo Kumenya

Salmonella PCR Kumenyekanisha Igikoresho cyerekanwe

Ibisobanuro bigufi:

Salmonella ni iya Enterobacteriaceae na Gram-negative enterobacteria.Salmonella ni indwara itera indwara kandi ikaza ku mwanya wa mbere mu kwangiza ibiryo bya bagiteri.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

izina RY'IGICURUZWA

Salmonella PCR Igikoresho cyo Kumenya (lyophilized)

Ingano

48ibizamini / ibikoresho, ibizamini 50 / ibikoresho

Gukoresha

Salmonella ni iya Enterobacteriaceae na Gram-negative enterobacteria.Salmonella ni indwara itera indwara kandi ikaza ku mwanya wa mbere mu kwangiza ibiryo bya bagiteri.Ibimenyetso nyamukuru byuburozi bwibiryo byatewe na Salmonella ni isesemi, kuruka, kubabara munda, kubabara umutwe, gukonja no gucibwamo.Iki gikoresho gikoresha ihame ryigihe-gihe cya fluorescent PCR kandi gikwiranye no kumenya neza Salmonella mubiribwa, ingero zamazi, umwanda, kuruka, hamwe namazi akungahaye.

Ibicuruzwa

Ibigize	Amapaki	Ibisobanuro	Ibikoresho
PCR Ivanga	1 icupa (Ifu ya Lyophilized)	50Ikizamini	DNTPs, MgCl₂, Primers, Ibibazo, Taq ADN polymerase
PCR Ivanga	6 × 0.2ml 8 umuyoboro mwiza(Lyophilized)	48Ikizamini	DNTPs, MgCl₂, Primers, Ibibazo, Taq ADN polymerase
Kugenzura neza	1 * 0.2ml umuyoboro (lyofilize)	10Ibizamini	Plasmid cyangwa Pseudovirus irimo ibice byihariye
Gukemura igisubizo	1.5 ml Cryotube	500uL	/
Kugenzura nabi	1.5 ml Cryotube	100uL	0.9% NaCl

Ububiko & Ubuzima bwa Shelf

(1) Igikoresho gishobora gutwarwa mubushyuhe bwicyumba.

(2) Ubuzima bwo kubaho ni amezi 18 kuri -20 ℃ n'amezi 12 kuri 2 ℃ ~ 30 ℃.

(3) Reba ikirango kuri kit kumunsi wumusaruro nitariki izarangiriraho.

Ibikoresho

GENECHECKER UF-150, UF-300 igikoresho nyacyo cya fluorescence PCR.

Igishushanyo mbonera

a) Icupa:

b) 8 verisiyo nziza ya tube:

Kwiyongera kwa Pcr

Gusabwa gushiraho

Intambwe	Ukuzenguruka	Ubushyuhe (℃)	Igihe	Umuyoboro wa Fluorescence
1	1	95	2min	/
2	40	95	5s	/
2	40	60	10s	Kusanya FAM fluorescence

Gusobanura ibisubizo by'ibizamini

Umuyoboro	Gusobanura ibisubizo
Umuyoboro wa FAM	Gusobanura ibisubizo
Ct≤35	Salmonella Nziza
Kureka	Salmonella
35	Gukeka gukekwa, gusubiramo *

* Niba ibisubizo byongeye kumuyoboro wa FAM bifite Ct agaciro ≤40 kandi byerekana imiterere isanzwe ya “S” amplification curve, ibisubizo bisobanurwa nkibyiza, naho ubundi nibibi.

Mbere: Norovirus (GⅡ) RT-PCR Igikoresho cyo Kumenya

Ibikurikira: Shigella PCR Kumenya