中文Vibrio parahaemolyticus PCR Kumenya Kit
izina RY'IGICURUZWA
Vibrio Parahaemolyticus PCR Kumenya Kit (Lyophilized)
Ingano
48ibizamini / ibikoresho, ibizamini 50 / ibikoresho
Gukoresha
Vibrio Parahemolyticus (izwi kandi ku izina rya Halophile Vibrio Parahemolyticus) ni Gram-negative polymorphic bacillus cyangwa Vibrio Parahemolyticus.ku gutangira gukabije, kubabara mu nda, kuruka, impiswi n'intebe y'amazi nk'ibimenyetso nyamukuru by'amavuriro.Iki gitabo gikoresha ihame rya fluorescent igihe nyacyo. PCR kandi ikwiranye no kumenya neza Vibrio Parahaemolyticus mu biribwa, ingero z’amazi, umwanda, kuruka, hamwe n’amazi akungahaye. Igikoresho ni SYSTEM YOSE YITEGUYE PCR (Lyophilized), ikubiyemo enzyme ya amplification ya ADN, reaction reaction, primers yihariye. nubushakashatsi bukenewe kugirango fluorescent RT-PCR imenyekane.
Ibicuruzwa
| Ibigize | Amapaki | Ibisobanuro | Ibikoresho |
| PCR Ivanga | 1 icupa (Ifu ya Lyophilized) | 50Ikizamini | DNTPs, MgCl2, Primers, Ibibazo, Taq ADN polymerase |
| 6 × 0.2ml 8 umuyoboro mwiza(Lyophilized) | 48Ikizamini | ||
| Kugenzura neza | 1 * 0.2ml umuyoboro (lyofilize) | 10Ibizamini | Plasmid cyangwa Pseudovirus irimo ibice byihariye |
| Gukemura igisubizo | 1.5 ml Cryotube | 500uL | / |
| Kugenzura nabi | 1.5 ml Cryotube | 100uL | 0.9% NaCl |
Ububiko & Ubuzima bwa Shelf
(1) Igikoresho gishobora gutwarwa mubushyuhe bwicyumba.
(2) Ubuzima bwo kubaho ni amezi 18 kuri -20 ℃ n'amezi 12 kuri 2 ℃ ~ 30 ℃.
(3) Reba ikirango kuri kit kumunsi wumusaruro nitariki izarangiriraho.
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Ibikoresho
GENECHECKER UF-150, UF-300 igikoresho nyacyo cya fluorescence PCR.
Igishushanyo mbonera
a) Icupa:
b) 8 verisiyo nziza ya tube:
Kwiyongera kwa Pcr
Gusabwa gushiraho
| Intambwe | Ukuzenguruka | Ubushyuhe (℃) | Igihe | Umuyoboro wa Fluorescence |
| 1 | 1 | 95 | 2min | / |
| 2 | 40 | 95 | 6s | / |
| 60 | 12s | Kusanya FAM fluorescence |
Gusobanura ibisubizo by'ibizamini
| Umuyoboro | Gusobanura ibisubizo |
| Umuyoboro wa FAM | |
| Ct≤35 | Vibrio Parahaemolyticus Nziza |
| Kureka | Vibrio Parahaemolyticus Ibibi |
| 35 | Gukeka gukekwa, gusubiramo * |
* Niba ibisubizo byongeye kumuyoboro wa FAM bifite Ct agaciro ≤40 kandi byerekana imiterere isanzwe ya “S” amplification curve, ibisubizo bisobanurwa nkibyiza, naho ubundi nibibi.
