中文E.coli O157: H7 PCR igikoresho cyo kumenya
izina RY'IGICURUZWA
E.coli O157: H7 PCR igikoresho cyo kumenya (Lyophilized)
Ingano
48ibizamini / ibikoresho, ibizamini 50 / ibikoresho
Gukoresha
Escherichia coli O157: H7 (E.coli O157: H7) ni bacteri ya garama-mbi yo mu bwoko bwa Enterobacteriaceae, itanga uburozi bwinshi bwa Vero.Mubuvuzi, mubisanzwe bibaho gitunguranye hamwe nububabare bukabije bwo munda hamwe nimpiswi zamazi, bikurikirwa nimpiswi ziva mumaraso nyuma yiminsi mike, zishobora gutera umuriro cyangwa kutagira umuriro, no gupfa mubihe bikomeye.Iki gikoresho gikwiranye no kumenya neza Escherichia coli O157: H7 mubiribwa, icyitegererezo cyamazi, umwanda, kuruka, amazi yongerera bagiteri nizindi ngero ukoresheje ihame ryigihe nyacyo PCR.Igitabo ni SYSTEM YOSE Yiteguye PCR ( Lyophilized), ikubiyemo enzyme ya ADN amplification enzyme, buffer reaction, primers na probe zisabwa kugirango fluorescent PCR ibone ..
Ibicuruzwa
| Ibigize | Amapaki | Ibisobanuro | Ibikoresho |
| E.coli O157: H7 PCR ivanze | 1 icupa (Ifu ya Lyophilized) | 50Ikizamini | DNTPs, MgCl2, Primers, Ibibazo, Guhindura Inyandiko, Taq ADN polymerase |
| 6 × 0.2ml 8 umuyoboro mwiza(Lyophilized) | 48Ikizamini | ||
| Kugenzura neza | 1 * 0.2ml umuyoboro (lyofilize) | 10Ibizamini | Plasmid irimo E.coli O157: H7 ibice byihariye |
| Gukemura igisubizo | 1.5 ml Cryotube | 500uL | / |
| Kugenzura nabi | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Ububiko & Ubuzima bwa Shelf
(1) Igikoresho gishobora gutwarwa mubushyuhe bwicyumba.
(2) Ubuzima bwo kubaho ni amezi 18 kuri -20 ℃ n'amezi 12 kuri 2 ℃ ~ 30 ℃.
(3) Reba ikirango kuri kit kumunsi wumusaruro nitariki izarangiriraho.
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Ibikoresho
GENECHECKER UF-150, UF-300 igikoresho nyacyo cya fluorescence PCR.
Igishushanyo mbonera
a) Icupa:
b) 8 verisiyo nziza ya tube:
Kwiyongera kwa Pcr
BasabweGushiraho
| Intambwe | Ukuzenguruka | Ubushyuhe (℃) | Igihe | Umuyoboro wa Fluorescence |
| 1 | 1 | 95 | 2min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Kusanya FAM fluorescence |
* Icyitonderwa: Ikimenyetso cyumuyoboro wa FAM fluorescence kizakusanywa kuri 60 ℃.
Gusobanura ibisubizo by'ibizamini
| Umuyoboro | Gusobanura ibisubizo |
| Umuyoboro wa FAM | |
| Ct≤35 | E.coli O157: H7 Ibyiza |
| Kureka | E.coli O157: H7 Ibibi |
| 35 | Gukeka gukekwa, gusubiramo * |
* Niba ibisubizo byongeye kumuyoboro wa FAM bifite Ct agaciro ≤40 kandi byerekana imiterere isanzwe ya “S” amplification curve, ibisubizo bisobanurwa nkibyiza, naho ubundi nibibi.
