Norovirus (GⅠ) RT-PCR Igikoresho cyo Kumenya

Ibisobanuro bigufi:

Birakwiriye kumenya Norovirus (GⅠ) muri shellfish, imboga mbisi n'imbuto, amazi, umwanda, kuruka nizindi ngero.Gukuramo aside nucleique bigomba gukorwa nuburyo bwo gukuramo aside nucleic cyangwa uburyo bwa pyrolysis butaziguye ukurikije ubwoko butandukanye.


Ibicuruzwa birambuye

Ibicuruzwa

izina RY'IGICURUZWA

Norovirus (GⅠ) RT-PCR Igikoresho cyo Kumenya (Lyophilized)

Ingano

48ibizamini / ibikoresho, ibizamini 50 / ibikoresho

Gukoresha

Birakwiriye kumenya Norovirus (GⅠ) muri shellfish, imboga mbisi n'imbuto, amazi, umwanda, kuruka nizindi ngero.Gukuramo aside nucleique bigomba gukorwa nigikoresho cyo gukuramo aside nucleic cyangwa uburyo bwa pyrolysis butaziguye ukurikije ubwoko butandukanye. Igikoresho ni SYSTEM YOSE YITEGUYE PCR (Lyophilized), ikubiyemo enzyme ya amplification ya ADN, reaction transcriptase, reaction buffer, primers yihariye nubushakashatsi bukenewe kugirango fluorescent PCR ibone.

Ibicuruzwa

Ibigize Amapaki Ibisobanuro Ibikoresho
Norovirus (GⅠ) PCR ivanze 1 icupa (Ifu ya Lyophilized)  50Ikizamini DNTPs, MgCl2, Primers, Ibibazo, Guhindura Inyandiko, Taq ADN polymerase
6 × 0.2ml 8 umuyoboro mwiza(Lyophilized) 48Ikizamini
Kugenzura neza 1 * 0.2ml umuyoboro (lyofilize)  10Ibizamini

Plasmid irimo Norovirus (GⅠ) ibice byihariye

Gukemura igisubizo 1.5 ml Cryotube 500uL /
Kugenzura nabi 1.5 ml Cryotube 200uL 0.9% NaCl

Ububiko & Ubuzima bwa Shelf

(1) Igikoresho gishobora gutwarwa mubushyuhe bwicyumba.

(2) Ubuzima bwo kubaho ni amezi 18 kuri -20 ℃ n'amezi 12 kuri 2 ℃ ~ 30 ℃.

(3) Reba ikirango kuri kit kumunsi wumusaruro nitariki izarangiriraho.

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Ibikoresho

GENECHECKER UF-150, UF-300 igikoresho nyacyo cya fluorescence PCR.

Igishushanyo mbonera

a) Icupa:

1

b) 8 verisiyo nziza ya tube:

2

Kwiyongera kwa Pcr

BasabweGushiraho

Intambwe Ukuzenguruka Ubushyuhe (℃) Igihe Umuyoboro wa Fluorescence
1 1 50 8min  
2 1 95 2min  
3 40 95 5s  
60 10s Kusanya FAM fluorescence

* Icyitonderwa: Ibimenyetso byumuyoboro wa FAM fluorescence bizakusanywa kuri 60 ℃.

Gusobanura ibisubizo by'ibizamini

Umuyoboro

Gusobanura ibisubizo

Umuyoboro wa FAM

Ct≤35

Norovirus (GⅠ) Ibyiza

Kureka

Norovirus (GⅠ) Ibibi

35

Gukeka gukekwa, gusubiramo *

* Niba ibisubizo byongeye kumuyoboro wa FAM bifite Ct agaciro ≤40 kandi byerekana imiterere isanzwe ya “S” amplification curve, ibisubizo bisobanurwa nkibyiza, naho ubundi nibibi.


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