中文Listeria monocytogène PCR Kumenya Kit
izina RY'IGICURUZWA
Listeria monocytogène PCR Kumenya Kit
Ingano
48ibizamini / ibikoresho, ibizamini 50 / ibikoresho
Gukoresha
Listeria monocytogene ni garama nziza ya microbacterium ishobora gukura hagati ya 4 ℃ na 45 ℃.Nimwe mumpamvu nyamukuru zitera ubuzima bwabantu mubiribwa bikonje.Ibyingenzi byingenzi byanduye ni septique, meningite na mononucleose.Iki gikoresho gikwiranye no kumenya neza Locytia monocytogène mu biribwa, icyitegererezo cy’amazi, umwanda, kuruka, amazi yongerera bagiteri nizindi ngero ukoresheje ihame rya fluorescence PC-nyayo. Igikoresho ni SYSTEM YOSE YITEGUYE (Lyophilized) , ikubiyemo enzyme ya amplification ya ADN, reaction transcriptase, reaction reaction, primers na probe zisabwa kugirango fluorescent PCR ibone.
Ububiko & Ubuzima bwa Shelf
(1) Igikoresho gishobora gutwarwa mubushyuhe bwicyumba.
(2) Ubuzima bwo kubaho ni amezi 18 kuri -20 ℃ n'amezi 12 kuri 2 ℃ ~ 30 ℃.
(3) Reba ikirango kuri kit kumunsi wumusaruro nitariki izarangiriraho.
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Ibicuruzwa
| Ibigize | Amapaki | Ibisobanuro | Ibikoresho |
| Urutonde rwa monocytogène PCR ivanze | 1 icupa (Ifu ya Lyophilized) | 50Ikizamini | DNTPs, MgCl2, Primers, Ibibazo, Guhindura Inyandiko, Taq ADN polymerase |
| 6 × 0.2ml 8 umuyoboro mwiza(Lyophilized) | 48Ikizamini | ||
| Kugenzura neza | 1 * 0.2ml umuyoboro (lyofilize) | 10Ibizamini | Plasmid irimo Listeria monocytogène ibice byihariye |
| Gukemura igisubizo | 1.5 ml Cryotube | 500uL | / |
| Kugenzura nabi | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Ibikoresho
GENECHECKER UF-150, UF-300 igikoresho nyacyo cya fluorescence PCR.
Igishushanyo mbonera
a)
b)
Kwiyongera kwa Pcr
BasabweGushiraho
| Intambwe | Ukuzenguruka | Ubushyuhe (℃) | Igihe | Umuyoboro wa Fluorescence |
| 1 | 1 | 50 | 8min | |
| 2 | 1 | 95 | 2min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Kusanya FAM fluorescence |
* Icyitonderwa: Ibimenyetso byumuyoboro wa FAM fluorescence bizakusanywa kuri 60 ℃.
Gusobanura ibisubizo by'ibizamini
| Umuyoboro | Gusobanura ibisubizo |
| Umuyoboro wa FAM | |
| Ct≤35 | Listeria monocytogène nziza |
| Kureka | Listeria monocytogène mbi |
| 35 | Gukeka gukekwa, gusubiramo * |
* Niba ibisubizo byongeye kumuyoboro wa FAM bifite Ct agaciro ≤40 kandi byerekana imiterere isanzwe ya “S” amplification curve, ibisubizo bisobanurwa nkibyiza, naho ubundi nibibi.
