中文Indwara ya porcine yimyororokere nubuhumekero virusi RT-PCR Detection Kit
izina RY'IGICURUZWA
Indwara ya porcine yimyororokere nubuhumekero virusi ya RT-PCR (Lyophilized)
Ingano
48ibizamini / ibikoresho, ibizamini 50 / ibikoresho
Gukoresha
Iki gikoresho gikoresha uburyo nyabwo bwa fluorescent RT-PCR kugirango hamenyekane RNA ya Porcine yimyororokere nubuhumekero syndrome nucleic aside detection kit (PRRSV) mubikoresho byindwara zifata nka toniilles, lymph node hamwe nintanga hamwe nibikoresho byindwara zamazi nkinkingo namaraso y'ingurube.Birakwiriye gutahura, gusuzuma no gukora epidemiologi ya virusi ya Porcine Blue Ear Virus.Igikoresho ni SYSTEM YOSE YITEGUYE PCR (Lyophilised), ikubiyemo transcript transcriptase, enzyme ya amplification ya ADN, reaction reaction, primers na probe zisabwa kugirango tumenye fluorescent RT-PCR.
Ibicuruzwa
| Ibigize | Amapaki | Ibisobanuro | Ibikoresho |
| PBEV PCR Ivanga | 1 icupa (Ifu ya Lyophilized) | 50Ikizamini | DNTPs, MgCl2, Primers, Ibibazo, Guhindura Inyandiko, Taq ADN polymerase |
| 6 × 0.2ml 8 umuyoboro mwiza(Lyophilized) | 48Ikizamini | ||
| Kugenzura neza | 1 * 0.2ml umuyoboro (lyofilize) | 10Ibizamini | Plasmid cyangwa Pseudovirus irimo PRRSV ibice byihariye |
| Gukemura igisubizo | 1.5 ml Cryotube | 500uL | / |
| Kugenzura nabi | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Ububiko & Ubuzima bwa Shelf
(1) Igikoresho gishobora gutwarwa mubushyuhe bwicyumba.
(2) Ubuzima bwo kubaho ni amezi 18 kuri -20 ℃ n'amezi 12 kuri 2 ℃ ~ 30 ℃.
(3) Reba ikirango kuri kit kumunsi wumusaruro nitariki izarangiriraho.
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Ibikoresho
GENECHECKER UF-150, UF-300 igikoresho nyacyo cya fluorescence PCR.
Igishushanyo mbonera
a) Icupa:
b) 8 verisiyo nziza ya tube:
Kwiyongera kwa Pcr
Gusabwa gushiraho
| Intambwe | Ukuzenguruka | Ubushyuhe (℃) | Igihe | Umuyoboro wa Fluorescence |
| 1 | 1 | 48 | 8min | / |
| 2 | 1 | 95 | 2min | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s | Kusanya FAM fluorescence |
* Icyitonderwa: Ibimenyetso byumuyoboro wa FAM fluorescence bizakusanywa kuri 60 ℃.
Gusobanura ibisubizo by'ibizamini
| Umuyoboro | Gusobanura ibisubizo |
| Umuyoboro wa FAM | |
| Ct≤35 | Vibrio Parahaemolyticus Nziza |
| Kureka | Vibrio Parahaemolyticus Ibibi |
| 35 | Gukeka gukekwa, gusubiramo * |
* Niba ibisubizo byongeye kumuyoboro wa FAM bifite Ct agaciro ≤40 kandi byerekana imiterere isanzwe ya “S” amplification curve, ibisubizo bisobanurwa nkibyiza, naho ubundi nibibi.
