中文Afurika y'ingurube virusi virusi PCR itahura ibikoresho
izina RY'IGICURUZWA
Afurika y'ingurube virusi ya PCR PCR igaragara (Lyophilized)
Ingano
48ibizamini / ibikoresho, ibizamini 50 / ibikoresho
Gukoresha
Iki gikoresho gikoresha uburyo bwa fluorescent PCR uburyo bwo kumenya ADN yaAfurika virusi y’ingurube (ASFV)mubikoresho byindwara zifata nka toni, lymph node hamwe nintanga nibikoresho byindwara zamazi nkinkingo namaraso yingurube.Birakwiriye gutahura, gusuzuma no gukora iperereza rya epidemiologiAfurika virusi y'ingurube.Igikoresho ni SYSTEM YOSE YITEGUYE PCR (Lyophilised), ikubiyemo enzyme ya amplification ya ADN, reaction reaction, primers na probe zisabwa kugirango tumenye fluorescent PCR ..
Ibicuruzwa
| Ibigize | Amapaki | Ibisobanuro | Ibikoresho |
| ASFV PCR ivanze | 1 icupa (Ifu ya Lyophilized) | 50Ikizamini | DNTPs, MgCl2, Primers, Ibibazo, Guhindura Inyandiko, Taq ADN polymerase |
| 6 × 0.2ml 8 umuyoboro mwiza(Lyophilized) | 48Ikizamini | ||
| Kugenzura neza | 1 * 0.2ml umuyoboro (lyofilize) | 10Ibizamini | Plasmid irimo ASFV ibice byihariye |
| Gukemura igisubizo | 1.5 ml Cryotube | 500uL | / |
| Kugenzura nabi | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Ububiko & Ubuzima bwa Shelf
(1) Igikoresho gishobora gutwarwa mubushyuhe bwicyumba.
(2) Ubuzima bwo kubaho ni amezi 18 kuri -20 ℃ n'amezi 12 kuri 2 ℃ ~ 30 ℃.
(3) Reba ikirango kuri kit kumunsi wumusaruro nitariki izarangiriraho.
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Ibikoresho
GENECHECKER UF-150, UF-300 igikoresho nyacyo cya fluorescence PCR.
Igishushanyo mbonera
a) Icupa:
b) 8 verisiyo nziza ya tube:
Kwiyongera kwa Pcr
BasabweGushiraho
| Intambwe | Ukuzenguruka | Ubushyuhe (℃) | Igihe | Umuyoboro wa Fluorescence |
| 1 | 1 | 95 | 2min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Kusanya FAM fluorescence |
* Icyitonderwa: Ibimenyetso byumuyoboro wa FAM fluorescence bizakusanywa kuri 60 ℃.
Gusobanura ibisubizo by'ibizamini
| Umuyoboro | Gusobanura ibisubizo |
| Umuyoboro wa FAM | |
| Ct≤35 | ASFV Nziza |
| Kureka | ASFV Ibibi |
| 35 | Ibisubizo biteye amakenga, gusubiramo * |
* Niba ibisubizo byongeye kumuyoboro wa FAM bifite Ct agaciro ≤40 kandi byerekana imiterere isanzwe ya “S” amplification curve, ibisubizo bisobanurwa nkibyiza, naho ubundi nibibi.
