中文Indwara yibirenge-umunwa virusi RT-PCR Detection Kit
izina RY'IGICURUZWA
Virusi yindwara yamaguru-umunwa RT-PCR Detection Kit (Lyophilized)
Ingano
48ibizamini / ibikoresho, ibizamini 50 / ibikoresho
Gukoresha
Iki gikoresho gikoresha uburyo nyabwo bwa fluorescent RT-PCR kugirango hamenyekane RNA yindwara yibirenge-umunwa (CSFV) mubikoresho byindwara zifata nka toniilles, lymph node na spleen nibikoresho byindwara zamazi nkurukingo namaraso yingurube.Birakwiriye gutahura, gusuzuma no gukora epidemiologi yindwara yibirenge.Igikoresho ni SYSTEM YOSE YITEGUYE PCR (Lyophilized), ikubiyemo enzyme ya amplification ya ADN, reaction transcriptase, reaction reaction, primers na probe zisabwa kugirango tumenye fluorescent PCR.
Ibicuruzwa
| Ibigize | Amapaki | Ibisobanuro | Ibikoresho |
| FMD PCR ivanze | 1 icupa (Ifu ya Lyophilized) | 50Ikizamini | DNTPs, MgCl2, Primers, Ibibazo, Guhindura Inyandiko, Taq ADN polymerase |
| 6 × 0.2ml 8 umuyoboro mwiza(Lyophilized) | 48Ikizamini | ||
| Kugenzura neza | 1 * 0.2ml umuyoboro (lyofilize) | 10Ibizamini | Plasmid irimo FMD ibice byihariye |
| Gukemura igisubizo | 1.5 ml Cryotube | 500uL | / |
| Kugenzura nabi | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Ububiko & Ubuzima bwa Shelf
(1) Igikoresho gishobora gutwarwa mubushyuhe bwicyumba.
(2) Ubuzima bwo kubaho ni amezi 18 kuri -20 ℃ n'amezi 12 kuri 2 ℃ ~ 30 ℃.
(3) Reba ikirango kuri kit kumunsi wumusaruro nitariki izarangiriraho.
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Ibikoresho
GENECHECKER UF-150, UF-300 igikoresho nyacyo cya fluorescence PCR.
Igishushanyo mbonera
a) Icupa:
b) 8 verisiyo nziza ya tube:
Kwiyongera kwa Pcr
BasabweGushiraho
| Intambwe | Ukuzenguruka | Ubushyuhe (℃) | Igihe | Umuyoboro wa Fluorescence |
| 1 | 1 | 50 | 8min | |
| 2 | 1 | 95 | 2min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Kusanya FAM fluorescence |
* Icyitonderwa: Ibimenyetso byumuyoboro wa FAM fluorescence bizakusanywa kuri 60 ℃.
Gusobanura ibisubizo by'ibizamini
| Umuyoboro | Gusobanura ibisubizo |
| Umuyoboro wa FAM | |
| Ct≤35 | FMD Ibyiza |
| Kureka | FMD Ibibi |
| 35 | Ibisubizo biteye amakenga, gusubiramo * |
* Niba ibisubizo byongeye kumuyoboro wa FAM bifite Ct agaciro ≤40 kandi byerekana imiterere isanzwe ya “S” amplification curve, ibisubizo bisobanurwa nkibyiza, naho ubundi nibibi.
