中文Staphylococcus aureus PCR Kumenya Kit
izina RY'IGICURUZWA
Staphylococcus aureus PCR Ikimenyetso cyo Kumenya (lyophilized)
Ingano
48ibizamini / ibikoresho, ibizamini 50 / ibikoresho
Gukoresha
Staphylococcus aureus ni iy'ubwoko bwa Staphylococcus kandi ni bagiteri nziza.Nibisanzwe biterwa na mikorobe mikorobe ishobora kubyara enterotoxine kandi igatera uburozi.Iki gikoresho gikoresha ihame ryigihe cya fluorescent PCR kandi gikwiranye no kumenya neza Staphylococcus aureus mubiribwa, icyitegererezo cyamazi, umwanda, kuruka, amazi akungahaye hamwe nizindi ngero.
Ibicuruzwa
| Ibigize | Amapaki | Ibisobanuro | Ibikoresho |
| PCR Ivanga | 1 icupa (Ifu ya Lyophilized) | 50Ikizamini | DNTPs, MgCl2, Primers, Ibibazo, Taq ADN polymerase |
| 6 × 0.2ml 8 umuyoboro mwiza(Lyophilized) | 48Ikizamini | ||
| Kugenzura neza | 1 * 0.2ml umuyoboro (lyofilize) | 10Ibizamini | Plasmid cyangwa Pseudovirus irimo ibice byihariye |
| Gukemura igisubizo | 1.5 ml Cryotube | 500uL | / |
| Kugenzura nabi | 1.5 ml Cryotube | 100uL | 0.9% NaCl |
Ububiko & Ubuzima bwa Shelf
(1) Igikoresho gishobora gutwarwa mubushyuhe bwicyumba.
(2) Ubuzima bwo kubaho ni amezi 18 kuri -20 ℃ n'amezi 12 kuri 2 ℃ ~ 30 ℃.
(3) Reba ikirango kuri kit kumunsi wumusaruro nitariki izarangiriraho.
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Ibikoresho
GENECHECKER UF-150, UF-300 igikoresho nyacyo cya fluorescence PCR.
Igishushanyo mbonera
a) Icupa:
b) 8 verisiyo nziza ya tube:
Kwiyongera kwa Pcr
Gusabwa gushiraho
| Intambwe | Ukuzenguruka | Ubushyuhe (℃) | Igihe | Umuyoboro wa Fluorescence |
| 1 | 1 | 95 | 2min | / |
| 2 | 40 | 95 | 6s | / |
| 60 | 12s | Kusanya FAM fluorescence |
Gusobanura ibisubizo by'ibizamini
| Channel | Gusobanura ibisubizo |
| FAMChannel | |
| Ct≤35 | Staphylococcus aureus Ibyiza |
| Kureka | Staphylococcus aureus Ibibi |
| 35<Ct≤40 | Gushidikanyaresut,retest * |
* Nibai gusubiramo ibisubizo byumuyoboro wa FAMhasagaciro Ct ≤40kandi yerekana bisanzwe“S”imiterere yo kongera umurongo, ibisubizo bisobanurwa nkibyiza, naho ubundi nibibi.
