中文Norovirus (GⅠ) RT-PCR Igikoresho cyo Kumenya
izina RY'IGICURUZWA
Norovirus (GⅠ) RT-PCR Igikoresho cyo Kumenya (Lyophilized)
Ingano
48ibizamini / ibikoresho, ibizamini 50 / ibikoresho
Gukoresha
Birakwiriye kumenya Norovirus (GⅠ) muri shellfish, imboga mbisi n'imbuto, amazi, umwanda, kuruka nizindi ngero.Gukuramo aside nucleique bigomba gukorwa nigikoresho cyo gukuramo aside nucleic cyangwa uburyo bwa pyrolysis butaziguye ukurikije ubwoko butandukanye. Igikoresho ni SYSTEM YOSE YITEGUYE PCR (Lyophilized), ikubiyemo enzyme ya amplification ya ADN, reaction transcriptase, reaction buffer, primers yihariye nubushakashatsi bukenewe kugirango fluorescent PCR ibone.
Ibicuruzwa
| Ibigize | Amapaki | Ibisobanuro | Ibikoresho |
| Norovirus (GⅠ) PCR ivanze | 1 icupa (Ifu ya Lyophilized) | 50Ikizamini | DNTPs, MgCl2, Primers, Ibibazo, Guhindura Inyandiko, Taq ADN polymerase |
| 6 × 0.2ml 8 umuyoboro mwiza(Lyophilized) | 48Ikizamini | ||
| Kugenzura neza | 1 * 0.2ml umuyoboro (lyofilize) | 10Ibizamini | Plasmid irimo Norovirus (GⅠ) ibice byihariye |
| Gukemura igisubizo | 1.5 ml Cryotube | 500uL | / |
| Kugenzura nabi | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Ububiko & Ubuzima bwa Shelf
(1) Igikoresho gishobora gutwarwa mubushyuhe bwicyumba.
(2) Ubuzima bwo kubaho ni amezi 18 kuri -20 ℃ n'amezi 12 kuri 2 ℃ ~ 30 ℃.
(3) Reba ikirango kuri kit kumunsi wumusaruro nitariki izarangiriraho.
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Ibikoresho
GENECHECKER UF-150, UF-300 igikoresho nyacyo cya fluorescence PCR.
Igishushanyo mbonera
a) Icupa:
b) 8 verisiyo nziza ya tube:
Kwiyongera kwa Pcr
BasabweGushiraho
| Intambwe | Ukuzenguruka | Ubushyuhe (℃) | Igihe | Umuyoboro wa Fluorescence |
| 1 | 1 | 50 | 8min | |
| 2 | 1 | 95 | 2min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Kusanya FAM fluorescence |
* Icyitonderwa: Ibimenyetso byumuyoboro wa FAM fluorescence bizakusanywa kuri 60 ℃.
Gusobanura ibisubizo by'ibizamini
| Umuyoboro | Gusobanura ibisubizo |
| Umuyoboro wa FAM | |
| Ct≤35 | Norovirus (GⅠ) Ibyiza |
| Kureka | Norovirus (GⅠ) Ibibi |
| 35 | Gukeka gukekwa, gusubiramo * |
* Niba ibisubizo byongeye kumuyoboro wa FAM bifite Ct agaciro ≤40 kandi byerekana imiterere isanzwe ya “S” amplification curve, ibisubizo bisobanurwa nkibyiza, naho ubundi nibibi.
