中文Virusi ya pseudorabies (gB) ibikoresho bya PCR
izina RY'IGICURUZWA
Virusi ya pseudorabies (gB) PCR Detection Kit (Lyophilized)
Ingano
48ibizamini / ibikoresho, ibizamini 50 / ibikoresho
Gukoresha
Iki gikoresho gikoresha uburyo nyabwo bwa fluorescent PCR kugirango hamenyekane RNA ya virusi ya Pseudorabies (gB gene) (PRV) mubikoresho byindwara zifata nka toniilles, lymph node na spleen hamwe nibikoresho byindwara zamazi nkinkingo namaraso yingurube.Birakwiriye gutahura, gusuzuma no gukora epidemiologi ya virusi ya Pseudorabies (gB gene).Igikoresho ni SYSTEM YOSE YITEGUYE PCR (Lyophilised), ikubiyemo enzyme ya amplification ya ADN, buffer reaction, primers na probe zisabwa kugirango fluorescent RT-PCR imenyekane.
Ibicuruzwa
| Ibigize | Amapaki | Ibisobanuro | Ibikoresho |
| PRV PCR Ivanga | 1 icupa (Ifu ya Lyophilized) | 50Ikizamini | DNTPs, MgCl2, Primers, Ibibazo, Taq ADN polymerase |
| 6 × 0.2ml 8 umuyoboro mwiza(Lyophilized) | 48Ikizamini | ||
| Kugenzura neza | 1 * 0.2ml umuyoboro (lyofilize) | 10Ibizamini | Plasmid cyangwa Pseudovirus irimo PRV (gB) ibice byihariye |
| Gukemura igisubizo | 1.5 ml Cryotube | 500uL | / |
| Kugenzura nabi | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Ububiko & Ubuzima bwa Shelf
(1) Igikoresho gishobora gutwarwa mubushyuhe bwicyumba.
(2) Ubuzima bwo kubaho ni amezi 18 kuri -20 ℃ n'amezi 12 kuri 2 ℃ ~ 30 ℃.
(3) Reba ikirango kuri kit kumunsi wumusaruro nitariki izarangiriraho.
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Ibikoresho
GENECHECKER UF-150, UF-300 igikoresho nyacyo cya fluorescence PCR.
Igishushanyo mbonera
a) Icupa:
b) 8 verisiyo nziza ya tube:
Kwiyongera kwa Pcr
Gusabwa gushiraho
| Intambwe | Ukuzenguruka | Ubushyuhe (℃) | Igihe | Umuyoboro wa Fluorescence |
| 1 | 1 | 95 | 2min | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s | Kusanya FAM fluorescence |
* Icyitonderwa: Ibimenyetso byumuyoboro wa FAM fluorescence bizakusanywa kuri 60 ℃.
Gusobanura ibisubizo by'ibizamini
| Umuyoboro | Gusobanura ibisubizo |
| Umuyoboro wa FAM | |
| Ct≤35 | PRV (gB) Ibyiza |
| Kureka | PRV (gB) Ibibi |
| 35 | Gukeka gukekwa, gusubiramo * |
* Niba ibisubizo byongeye kumuyoboro wa FAM bifite Ct agaciro ≤40 kandi byerekana imiterere isanzwe ya “S” amplification curve, ibisubizo bisobanurwa nkibyiza, naho ubundi nibibi.
