中文Virusi y'ingurube RT-PCR Kumenya
izina RY'IGICURUZWA
Virusi y'ingurube RT-PCR Detection Kit (Lyophilized)
Ingano
48ibizamini / ibikoresho, ibizamini 50 / ibikoresho
Gukoresha
Iki gikoresho gikoresha uburyo nyabwo bwa fluorescent RT-PCR kugirango hamenyekane RNA ya virusi ya Swine fever (CSFV) mubikoresho byindwara zifata nka toniilles, node ya lymph na spleen nibikoresho byindwara zamazi nkurukingo namaraso yingurube.Birakwiriye gutahura, gusuzuma no gukora epidemiologi ya virusi yumuriro wingurube.Igikoresho ni SYSTEM YOSE YITEGUYE PCR (Lyophilized), ikubiyemo enzyme ya amplification ya ADN, reaction transcriptase, reaction reaction, primers na probe zisabwa kugirango tumenye fluorescent PCR.
Ibicuruzwa
| Ibigize | Amapaki | Ibisobanuro | Ibikoresho |
| CSFV PCR ivanze | 1 icupa (Ifu ya Lyophilized) | 50Ikizamini | DNTPs, MgCl2, Primers, Ibibazo, Guhindura Inyandiko, Taq ADN polymerase |
| 6 × 0.2ml 8 umuyoboro mwiza(Lyophilized) | 48Ikizamini | ||
| Kugenzura neza | 1 * 0.2ml umuyoboro (lyofilize) | 10Ibizamini | Plasmid irimo CSFV ibice byihariye |
| Gukemura igisubizo | 1.5 ml Cryotube | 500uL | / |
| Kugenzura nabi | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Ububiko & Ubuzima bwa Shelf
(1) Igikoresho gishobora gutwarwa mubushyuhe bwicyumba.
(2) Ubuzima bwo kubaho ni amezi 18 kuri -20 ℃ n'amezi 12 kuri 2 ℃ ~ 30 ℃.
(3) Reba ikirango kuri kit kumunsi wumusaruro nitariki izarangiriraho.
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Ibikoresho
GENECHECKER UF-150, UF-300 igikoresho nyacyo cya fluorescence PCR.
Igishushanyo mbonera
a) Icupa:
b) 8 verisiyo nziza ya tube:
Kwiyongera kwa Pcr
BasabweGushiraho
| Intambwe | Ukuzenguruka | Ubushyuhe (℃) | Igihe | Umuyoboro wa Fluorescence |
| 1 | 1 | 50 | 8min | |
| 2 | 1 | 95 | 2min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Kusanya FAM fluorescence |
* Icyitonderwa: Ibimenyetso byumuyoboro wa FAM fluorescence bizakusanywa kuri 60 ℃.
Gusobanura ibisubizo by'ibizamini
| Umuyoboro | Gusobanura ibisubizo |
| Umuyoboro wa FAM | |
| Ct≤35 | CSFV Ibyiza |
| Kureka | CSFV Ibibi |
| 35 | Ibisubizo biteye amakenga, gusubiramo * |
* Niba ibisubizo byongeye kumuyoboro wa FAM bifite Ct agaciro ≤40 kandi byerekana imiterere isanzwe ya “S” amplification curve, ibisubizo bisobanurwa nkibyiza, naho ubundi nibibi.
